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qPCR and qRT-PCR

  • TransStart<sup>®</sup>  Probe qPCR SuperMix
TransStart<sup>®</sup>  Probe qPCR SuperMix

TransStart® Probe qPCR SuperMix

  • Product Introduction
  • Manual Download
  • References


TransStart® Probe qPCR SuperMix is a ready-to-use qPCR cocktail containing all components, except probe, primer and template. It contains TransStart® Taq DNA Polymerase, dNTPs, PCR enhancer and stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, probe, passive reference dye (optional) and ddH2O.


TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and
generates more accurate data.
• Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
• Passive reference dyes are provided for different qPCR instruments.

Passive Reference

Dye Selection

• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q


at -20°C in dark for one year

Kit Contents





TransStart® Probe qPCR SuperMix

1 ml

5×1 ml

15×1 ml

Passive Reference Dye (50×)

40 μl

200 μl

600 μl


1 ml

5 ml

3×5 ml

Literature Journal IF Author Date Link