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qPCR and qRT-PCR

  • TransScript<sup>®</sup> II Green Two-Step qRT-PCR SuperMix
TransScript<sup>®</sup> II Green Two-Step qRT-PCR SuperMix

TransScript® II Green Two-Step qRT-PCR SuperMix


  • Product Introduction
  • Manual Download
  • References

Description

TransScript® II Green Two-Step qRT-PCR SuperMix contains all the necessary reagents for gDNA removal, cDNA synthesis and qPCR.

Highlights

• gDNA remover and 5×TransScript® II All-in-One SuperMix for qPCR are provided for simultaneous gDNA removal and cDNA synthesis.
• TransStart® Tip Green qPCR SuperMix is provided for qPCR.
• 5×TransScript® II All-in-One No-RT Control SuperMix for qPCR is provided for experimental control.
• Passive reference dyes are provided for different qPCR instruments.

Applications

• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

Passive Reference

Dye Selection

• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q

Storage

at -20°C in dark for one year

Kit Contents

Component

AQ301-01

5×TransScript® II All-in-One SuperMix for qPCR

200 μl

5×TransScript® II All-in-One No-RT Control SuperMix for qPCR

20 μl

gDNA Remover

50 μl

TransStart® Tip Green qPCR SuperMix (2×)

3×1 ml

Passive Reference Dye (50×)

120 μl

RNase-free Water

1 ml




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