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qPCR and qRT-PCR

  • TransScript<sup>®</sup> Green miRNA Two-Step qRT-PCR SuperMix
TransScript<sup>®</sup> Green miRNA Two-Step qRT-PCR SuperMix

TransScript® Green miRNA Two-Step qRT-PCR SuperMix

  • Product Introduction
  • Manual Download
  • References


TransScript® Green miRNA Two-Step qRT-PCR SuperMix provides all the necessary components for miRNA detection. High efficient poly(A) tail addition and first-strand cDNA synthesis are performed by TransScript® miRNA RT Enzyme Mix (containing tailing enzyme and RT enzyme) and 2×TS miRNA Reaction Mix. TransScript® Tip Green qPCR SuperMix is provided for miRNA detection.


• One-Step poly(A) tailing and cDNA synthesis.
• Passive reference dyes are provided for different qPCR instruments.


Multiple copy and low copy gene detection

Passive Reference

Dye Selection

• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q


at -20°C in dark for one year

Kit Contents



TransScript® miRNA RT Enzyme Mix

20 μl

2×TS miRNA Reaction Mix

200 μl

Universal miRNA qPCR Primer (10 μM)

200 μl

2×TransStart® Tip Green qPCR SuperMix

5×1 ml

Passive Reference Dye (50×)

200 μl

RNase-free Water

1 ml

Literature Journal IF Author Date Link