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RT-PCR

  • Rinbonuclease Inhibitor
  • Rinbonuclease Inhibitor
Rinbonuclease InhibitorRinbonuclease Inhibitor

Rinbonuclease Inhibitor


  • Product Introduction
  • Manual Download
  • References

Description

Ribonuclease Inhibitor is a recombinant protein purified from E. coli strain carrying human placenta ribonuclease inhibitor gene. Ribonuclease Inhibitor specifically inhibits RNase A, RNase B, and RNase C. It is not effective against RNase 1, RNase T1, S1 nuclease, RNase H and aspergillus-originated RNase. It has no inhibition effect on DNA Polymerase, AMV, M-MLV, SP6, T7 and T3 RNA Polymerases.


Storage Buffers

50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 10 mM DTT, 100 mM NaCl, 50% glycerol


Unit Definition

One unit is defined as the amount of enzyme required to inhibit 5 ng RNase A by 50%.


Applications

In vitro inhibition of ribonuclease, cDNA synthesis and in vitro transcription and translation.


Storage

at -20℃ for two year


Reaction Components


Component

Volume

Total RNA/mRNA

50 ng-5 μg/5-500 ng

Anchored Oligo(dT)18 Primer (0.5 μg/μl)

1 μl

or Random Primer (0.1 μg/μl)

1 μl

or GSP

2 pmol

10 mM dNTPs

1 μl

5× RT Buffer

4 μl

Ribonuclease Inhibitor

0.5 μl

EasyScript®/TransScript® RT

1 μl



Literature Journal IF Author Date Link
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