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RT-PCR

  • TransScript<sup>®</sup>  II All-in-One First-Strand cDNA Synthesis SuperMix for PCR
TransScript<sup>®</sup>  II All-in-One First-Strand cDNA Synthesis SuperMix for PCR

TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for PCR


  • Product Introduction
  • Manual Download
  • References

Description

TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. The SuperMix is provided at 5× concentration and used at 1× concentration by adding RNA and H2O. The resulting cDNA is suitable for regular PCR, not for qPCR.

Highlights

• One-tube format for simple and fast setup and reduced pipetting variability.
• The optimal ratio of oligo(dT)20 primer to random primer(N9) for PCR ready cDNA.
• PCR ready cDNA in 30 minutes (unsuitable for qPCR).
• cDNA up to 15 kb.

Applications

• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

Storage

at -20℃ for two year

Kit Contents



Components

AH321-01 (50 rxns )

5×TransScript® II All-in-One SuperMix for PCR

200 μl

RNase-free Water

1 ml


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