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RT-PCR

  • TransScript<sup>®</sup> All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )
TransScript<sup>®</sup> All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )

TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )


  • Product Introduction
  • Manual Download
  • References


Description

The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. The resulting cDNA is suitable for qPCR, not for regular PCR.

Highlights

• Simultaneous genomic DNA removal and cDNA synthesis.
• The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
• qPCR ready cDNA in 15 minutes.
• cDNA up to 250 bp.

Applications

Multiple copy and low copy gene detection

Storage

at -20℃ for two year

Kit Contents



Components

AT341-01 (50 rxns )

AT341-02 (100 rxns )

5×TransScript® All-in-One SuperMix for qPCR

200 μl

400 μl

5×TransScript® All-in-One No-RT Control SuperMix for qPCR

20 μl

40 μl

gDNA Remover

50 μl

100 μl

RNase-free Water

1 ml

2×1 ml

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