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RT-PCR

  • TransScript<sup>®</sup>  All-in-One First-Strand cDNA Synthesis SuperMix for PCR
TransScript<sup>®</sup>  All-in-One First-Strand cDNA Synthesis SuperMix for PCR

TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for PCR


  • Product Introduction
  • Manual Download
  • References

Description

TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. The SuperMix is provided at 5× concentration and used at 1× concentration by adding RNA and H2O. The resulting cDNA are suitable for regular PCR, not for qPCR.


Highlights

• One-tube format for simple and fast setup and reduce pipetting variability.
• The optimal ratio of oligo(dT)18 primer to random primer(N9) for PCR-ready cDNA.
• PCR-ready cDNA in 30 minutes (unsuitable for qPCR).
• cDNA up to 12 kb.


Applications

Multiple copy and low copy gene detection


Storage

at -20℃ for two year


Shipping Condition

Dry ice(-70℃)


Kit Contents



Component

AT321-01 (50 rxns)

5×TransScript® All-in-One SuperMix for PCR

200 μl

RNase-free Water

1 ml


Literature Journal IF Author Date Link

CONTACT US

Contacts:Shuyi Yan

Skype:TransGen Biotech Co., LTD

Telephone:0086-10-57815027

E-mail:info@transgenbiotech.com

Address: Building No.4, Zhongguancun Dongsheng International Science Park, 1 North Yongtaizhuang Road, Haidian District, 100192 Beijing, China


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