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RT-PCR

  • TransScript<sup>®</sup> II One-Step gDNA Removal and  cDNA Synthesis SuperMix
TransScript<sup>®</sup> II One-Step gDNA Removal and  cDNA Synthesis SuperMix

TransScript® II One-Step gDNA Removal and cDNA Synthesis SuperMix


  • Product Introduction
  • Manual Download
  • References

Description

Unique genomic DNA remover is combined with TransScript® II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.

Highlights

• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 15 kb.

Applications

• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

Storage

at -20℃ for two year

Shipping Condition

Dry ice(-70℃)

Kit Contents

Component

AH311-02 (50 rxns)

AH311-03 (100 rxns)

TransScript® II RT/RI Enzyme Mix

50 μl

100 μl

gDNA Remover

50 μl

100 μl

2×TS II Reaction Mix

500 μl

1 ml

Random Primer (0.1 μg/μl)

50 μl

100 μl

Anchored Oligo(dT)20 Primer (0.5 μg/μl)

50 μl

100 μl

RNase-free Water

500 μl

1 ml




Literature Journal IF Author Date Link

CONTACT US

Contacts:Shuyi Yan

Skype:TransGen Biotech Co., LTD

Telephone:0086-10-57815027

E-mail:info@transgenbiotech.com

Address: Building No.4, Zhongguancun Dongsheng International Science Park, 1 North Yongtaizhuang Road, Haidian District, 100192 Beijing, China


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