Hello,Welcome:           Login line Register

line Shopping Cart line My orders line Chinese line

RT-PCR

  • TransScript<sup>®</sup>  One-Step gDNA Removal and  cDNA Synthesis SuperMix
TransScript<sup>®</sup>  One-Step gDNA Removal and  cDNA Synthesis SuperMix

TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix


  • Product Introduction
  • Manual Download
  • References


Description

Unique genomic DNA remover is combined with TransScript® First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.

Highlights

• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 12 kb.

Applications

Multiple copy and low copy gene detection

Storage

at -20℃ for two year

Kit Contents



Component

AT311-02 (50 rxns)

AT311-03 (100 rxns)

TransScript® RT/RI Enzyme Mix

50 μl

100 μl

gDNA Remover

50 μl

100 μl

2×TS Reaction Mix

500 μl

1 ml

Random Primer(N9) (0.1 μg/μl)

50 μl

100 μl

Anchored Oligo(dT)18 Primer (0.5 μg/μl)

50 μl

100 μl

RNase-free Water

500 μl

1 ml



Literature Journal IF Author Date Link
Message