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RT-PCR

  • TransScript<sup>®</sup>  First-Strand cDNA Synthesis SuperMix
TransScript<sup>®</sup>  First-Strand cDNA Synthesis SuperMix

TransScript® First-Strand cDNA Synthesis SuperMix


  • Product Introduction
  • Manual Download
  • References


Description

TransScript First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript RT/RI Enzyme Mix and 2xTS Reaction Mix.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 12 kb.


Applications

Multiple copy and low copy gene detection


Storage


at -20℃ for two year


Kit Contents


Component

AT301-02

AT301-03

TransScript® RT/RI Enzyme Mix

50 µl

100 µl

2×TS Reaction Mix

500 µl

1 ml

Random Primer (N9) (0.1 µg/µl)

50 µl

100 µl

Anchored Oligo (dT)18 Primer (0.5 µg/µl)

50 µl

100 µl

RNase-free Water

500 µl

1 ml



Literature Journal IF Author Date Link

CONTACT US

Contacts:Shuyi Yan

Skype:TransGen Biotech Co., LTD

Telephone:0086-10-57815027

E-mail:info@transgenbiotech.com

Address: Building No.4, Zhongguancun Dongsheng International Science Park, 1 North Yongtaizhuang Road, Haidian District, 100192 Beijing, China


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