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RT-PCR

  • EasyScript First-Strand cDNA Synthesis SuperMix
EasyScript First-Strand cDNA Synthesis SuperMix

EasyScript First-Strand cDNA Synthesis SuperMix


  • Product Introduction
  • Manual Download
  • References

Description

EasyScript® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by EasyScript® RT/RI Enzyme Mix and 2×ES Reaction Mix.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the, product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 8 kb.

Applications

Multiple copy gene detection

Storage

at -20℃ for two year

Kit Contents



Component

AE301-02

AE301-03

EasyScript® RT/RI Enzyme Mix

50 µl

100 µl

2×ES Reaction Mix

500 µl

1 ml

Random Primer(N9) (0.1 µg/µl)

50 µl

100 µl

Anchored Oligo(dT)18 Primer (0.5 µg/µl)

50 µl

100 µl

RNase-free Water

500 µl

1 ml



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