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RT-PCR

  • TransScript<sup>®</sup> II Reverse Transcriptase
TransScript<sup>®</sup> II Reverse Transcriptase

TransScript® II Reverse Transcriptase


  • Product Introduction
  • Manual Download
  • References

Description

TransScript® II Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability. The enzyme is active at up to 55℃. It provides higher specificity, higher yield and more full-length cDNA products.
• Increased thermostability for more full-length cDNA products.
• Reaction temperature at 42℃-55℃.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
• cDNA up to 15 kb.

Applications

• First-strand cDNA synthesis, cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

Unit Definition

One unit of TransScript® II RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37℃ using Poly(A)/Oligo(dT) as template/primer.

10×TS II RT Buffer

500 mM KCl, 30 mM MgCl2, 100 mM Tris-HCl pH 8.4

Storage

at -20℃ for two year

Shipping Condition

Dry ice (-70 )

Kit Contents



Component

AH101-02

TransScript® II RT

10000 U

10×TS II RT Buffer

100 μl

Anchored Oligo(dT)20 Primer (0.5 µg/µl)

50 μl


Literature Journal IF Author Date Link

CONTACT US

Contacts:Shuyi Yan

Skype:TransGen Biotech Co., LTD

Telephone:0086-10-57815027

E-mail:info@transgenbiotech.com

Address: Building No.4, Zhongguancun Dongsheng International Science Park, 1 North Yongtaizhuang Road, Haidian District, 100192 Beijing, China


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