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PCR SuperMix

  • 2×TransStart FastPfu Fly PCR SuperMix
2×TransStart FastPfu Fly PCR SuperMix

2×TransStart FastPfu Fly PCR SuperMix


  • Product Introduction
  • Manual Download
  • References

Description

TransStart® FastPfu Fly PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu Fly DNA polymerase, dNTPs, and optimized buffer. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.

• TransStart® FastPfu Fly PCR SuperMix offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 2-6 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.

Applications

• High fidelity PCR
• High yield PCR
• GC-rich  PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates

Storage

at -20oC for two years

Kit Contents

Component

AS231-01

AS231-02

2×TransStart® FastPfu Fly PCR SuperMix (-dye)

1 ml

5×1 ml

ddH2O

1 ml

5 ml

Reaction System

Component

Volume

Final Concentration

Template

Variable

as required

Forward Primer (10 μM)

1 μl

0.2 μM

Reverse Primer (10 μM)

1 μl

0.2 μM

2×TransStart® FastPfu Fly PCR SuperMix

25 μl

1x

ddH2O

Variable

-

Total volume

50 μl

-




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