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PCR SuperMix

  • 2×TransStart<sup>®</sup> FastPfu PCR SuperMix (-dye )
2×TransStart<sup>®</sup> FastPfu PCR SuperMix (-dye )

2×TransStart® FastPfu PCR SuperMix (-dye )


  • Product Introduction
  • Manual Download
  • References

Description

TransStart® FastPfu PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu DNA polymerase, dNTPs, and optimized buffer. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.

• TransStart® FastPfu PCR SuperMix offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 2-4 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.

Applications

• High fidelity PCR
• High yield PCR
• Fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates

Storage

at -20℃ for two years

Kit Contents

Component

AS221-01

AS221-02

2×TransStart® FastPfu PCR SuperMix (-dye)

1 ml

5×1 ml

ddH2O

1 ml

5 ml

Reaction System

Component

Volume

Final Concentration

Template

Variable

As required

Forward Primer (10 μM)

1 μl

0.2 μM

Reverse Primer (10 μM)

1 μl

0.2 μM

2×TransStart® FastPfu PCR SuperMix

25 μl

ddH2O

Variable

-

Total volume

50 μl

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