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PCR SuperMix

  • 2×EasyPfu<sup>®</sup> PCR SuperMix (-dye )
  • 2×EasyPfu<sup>®</sup> PCR SuperMix (-dye )
2×EasyPfu<sup>®</sup> PCR SuperMix (-dye )2×EasyPfu<sup>®</sup> PCR SuperMix (-dye )

2×EasyPfu® PCR SuperMix (-dye )


  • Product Introduction
  • Manual Download
  • References


Description

2×EasyPfu PCR SuperMix is a ready-to-use mixture of EasyPfu DNA Polymerase, dNTPs and optimized buffer. The SuperMix is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O.

• EasyPfu PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.

• Extension rate is about 0.5 kb/min.

• PCR products can be directly cloned into pEASY®-Blunt vectors.

• Amplification of genomic DNA fragment up to 6 kb.

• Amplification of plasmid DNA fragment up to 10 kb.

Applications

• High fidelity PCR
• Blunt end cloning
• Site-directed mutagenesis

Storage

at -20℃ for two years

Kit Contents

Component

AS211-01

AS211-02

2×EasyPfu PCR SuperMix (-dye)

1 ml

5×1 ml

ddH2O

1 ml

5 ml

Reaction System

Component

Volume

Final Concentration

Template

Variable

As required

Forward Primer (10 μM)

1 μl

0.2 μM

Reverse Primer (10 μM)

1 μl

0.2 μM

2×EasyPfu PCR SuperMix

25 μl

ddH2O

Variable

-

Total volume

50 μl

-



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