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PCR SuperMix

  • 2×TransTaq<sup>®</sup> High Fidelity(HiFi)PCR SuperMix I (-dye )
2×TransTaq<sup>®</sup> High Fidelity(HiFi)PCR SuperMix I (-dye )

2×TransTaq® High Fidelity(HiFi)PCR SuperMix I (-dye )


  • Product Introduction
  • Manual Download
  • References

Description

TransTaq® High Fidelity (HiFi) PCR SuperMix I is a ready-to-use mixture of TransTaq® High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer. TransTaq® High Fidelity (HiFi) PCR SuperMix I is optimized for the amplification of genomic DNA. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.

TransTaq®High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.


Applications

• Complex templates
• GC/AT-rich templates
• Long PCR
• High yield PCR


Storage

at -20℃ for two years


Kit Contents


Component

AS131-01

AS131-02

TransTaq® HiFi PCR SuperMix I

1 ml

5×1 ml

ddH2O

1 ml

5 ml


Reaction System


Component

Volume

Final Concentration

Template

Variable

As required

Forward Primer (10 μM)

1 μl

0.2 μM

Reverse Primer (10 μM)

1 μl

0.2 μM

TransTaq® HiFi PCR SuperMix I

25 μl

ddH2O

Variable

-

Total volume

50 μl

-




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