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PCR Enzyme

  • TransStart<sup>®</sup> KD Plus DNA Polymerase
  • TransStart<sup>®</sup> KD Plus DNA Polymerase
TransStart<sup>®</sup> KD Plus DNA PolymeraseTransStart<sup>®</sup> KD Plus DNA Polymerase

TransStart® KD Plus DNA Polymerase


  • Product Introduction
  • Manual Download
  • References

Description

TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase. This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3’-5’ exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.

Highlights

• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.

Applications

• Fast, high specificity amplification
• High fidelity, high yield amplification

Storage Buffer

50 mM Tris-Cl (pH 8.0)50 mM KCl1 mM DTT0.1 mM EDTAStabilizers50% glycerol.

Storage

at -20 ℃ for two years

Kit Contents

Component

AP301-01/11

AP301-02/12

AP301-03/13

TransStart® KD Plus DNA Polymerase

100 U×1

200 U×1

200 U×6

5×TransStart® KD Plus Buffer

1.2 ml×1

1.2 ml ×2

1.2 ml ×12

2.5 mM dNTPs

- / 500 µl×1

- / 1 ml ×1

- / 1 ml ×6

50 mM MgSO4

200 µl×1

400 µl ×1

1 ml ×1

6×DNA Loading Buffer

500 μl×1

1 ml ×1

1 ml ×2

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