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PCR Enzyme

  • TransStart<sup>®</sup> FastPfu Fly DNA Polymerase
TransStart<sup>®</sup> FastPfu Fly DNA Polymerase

TransStart® FastPfu Fly DNA Polymerase

  • Product Introduction
  • Manual Download
  • References


TransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase. TransStart® FastPfuFly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart®FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.


• TransStart® FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 2-6 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.


• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates

Unit Definition

One unit of TransStart® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

Quality Control

TransStart® FastPfu Fly DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu Fly DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Storage Buffer

50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% glycerol


at -20 ℃ for two years

Kit Contents





TransStart® FastPfu Fly DNA Polymerase

250 U×1

500 U×1

500 U×6

5×TransStart® FastPfu Fly Buffer

1.2 ml×1

1.2 ml ×2

1.2 ml ×12

2.5 mM dNTPs

- / 500 μl×1

- / 1 ml ×1

- / 1 ml ×6

50 mM MgSO4

200 µl×1

400 µl ×1

1 ml ×1

PCR Stimulant

200 µl×1

400 µl×1

1 ml ×1

6×DNA Loading Buffer

500 μl×1

1 ml ×1

1 ml ×2

Related Images

Literature Journal IF Author Date Link