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PCR Enzyme

  • EasyPfu<sup>®</sup> DNA Polymerase
  • EasyPfu<sup>®</sup> DNA Polymerase
  • EasyPfu<sup>®</sup> DNA Polymerase
  • EasyPfu<sup>®</sup> DNA Polymerase
  • EasyPfu<sup>®</sup> DNA Polymerase
  • EasyPfu<sup>®</sup> DNA Polymerase
EasyPfu<sup>®</sup> DNA PolymeraseEasyPfu<sup>®</sup> DNA PolymeraseEasyPfu<sup>®</sup> DNA PolymeraseEasyPfu<sup>®</sup> DNA PolymeraseEasyPfu<sup>®</sup> DNA PolymeraseEasyPfu<sup>®</sup> DNA Polymerase

EasyPfu® DNA Polymerase


  • Product Introduction
  • Manual Download
  • References

Description

EasyPfu DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity. EasyPfu DNAPolymerase possesses a proofreading 3'-5' exonuclease activity.

Highlights

• EasyPfu DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 0.5 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 6 kb.
• Amplification of plasmid DNA fragment up to 10 kb.

Applications

• High fidelity PCR
• Blunt-end cloning
• Site-directed mutagenesis

Unit Definition

One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

Quality Control

EasyPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of EasyPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Storage Buffer

50 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol stabilizers

Storage

at -20°C for two years

Shipping Condition

Dry ice (-70 ℃)


Kit Contents


Component

AP211-01/11

AP211-02/12

AP211-03/13

EasyPfu DNA Polymerase

250 U×1

500 U×1

500 U×6

10×EasyPfu Buffer

1.2 ml ×1

1.2 ml ×1

1.2 ml ×6

2.5 mM dNTPs

- / 500 µl ×1

- / 1 ml ×1

- / 1 ml ×6

6×DNA Loading Buffer

500 μl×1

1 ml ×1

1 ml ×2

50 mM MgSO4

200 μl×1

400 μl×1

1 ml ×1


Related Images





Literature Journal IF Author Date Link

CONTACT US

Contacts:Shuyi Yan

Skype:TransGen Biotech Co., LTD

Telephone:0086-10-57815027

E-mail:info@transgenbiotech.com

Address: Building No.4, Zhongguancun Dongsheng International Science Park, 1 North Yongtaizhuang Road, Haidian District, 100192 Beijing, China


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