• Do not use pluripotent stem cell culture medium to dilute the 500×AP Live Solution. Do not observe the result in pluripotent stem
cell culture medium.
• Since AP is ubiquitously expressed in most cell types and highest in pluripotent stem cells. It is normal that dim staining can be
observed in some somatic cells such as CHO cells, HeLa cells and cells from the placenta, and so on.
• After removal of dye from the media, fluorescently labeled cells lose their signal by exocytosis in 1.5-2 hours, please observe the
results within 1 hour after staining.
• Please avoid repeated freezing and thawing. When necessary, the working concentration can be increased.