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Cell Detection

  • TransDetect<sup>®</sup> Annexin V-FITC/PI Cell  Apoptosis Detection Kit
TransDetect<sup>®</sup> Annexin V-FITC/PI Cell  Apoptosis Detection Kit

TransDetect® Annexin V-FITC/PI Cell Apoptosis Detection Kit


  • Product Introduction
  • Manual Download
  • References

Description

The Annexin V-FITC/PI Cell Apoptosis Kit provides a rapid and sensitive method for early apoptosis detection. In normal cells, the membrane phospholipid phosphatidylserine (PS) is located on the cytoplasmic surface of the membrane. In apoptotic cells, PS is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. The FITC-conjugated Annexin V, a Ca2+ dependent phospholipid-binding protein, can bind specifically to the exposed PS. Propidium iodide (PI) is a nucleic acid binding dye, which binds tightly to the nucleic acids in the cells and stains the cells with red fluorescence.PI is impermeant to live cells and early apoptotic cells, so the combination of Annexin V-FITC and PI staining allows the differentiation among different stage of apoptotic cells and necrosis cells.


Storage

at 4°C in dark for one year.


Kit Content


Component

FA101-01 (25 rxns)

FA101-02 (50 rxns)

Annexin V-FITC

125 μl

250 μl

Propidium Iodide (PI)

125 μl

250 μl

1× Annexin V Binding Buffer

12.5 ml

2×12.5 ml



Literature Journal IF Author Date Link
Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells Oncotarget 6.359 Chengle Zhuang, et al. 2015 Oct The original link
Carboxyamidotriazole inhibits oxidative phosphorylation in cancer cells and exerts synergistic anti-cancer effect with glycolysis inhibition Cancer Letters 5.621 Rui Ju, et al. 2015 Oct The original link
Acetylation of p53 at lysine 120 upregulates Apaf-1 and sensitizes mitochondrial apoptotic pathway JOURNAL OF BIOLOGICAL CHEMISTRY 4.573 Tao Yun, et al. 2016 Feb The original link
Intracellular calcium promotes radioresistance of non?Csmall cell lung cancer A549 cells through activating Akt signaling tumor biology 3.65 Yiling Wang,et al. 2017Mar The original link
FIP-sch2, a new fungal immunomodulatory protein from Stachybotrys chlorohalonata, suppresses proliferation and migration in lung cancer cells applied microbiology and biotechnology 3.42 Shuying Li,et al. 2017Apr The original link

CONTACT US

Contacts:Shuyi Yan

Skype:TransGen Biotech Co., LTD

Telephone:0086-10-57815027

E-mail:info@transgenbiotech.com

Address: Building No.4, Zhongguancun Dongsheng International Science Park, 1 North Yongtaizhuang Road, Haidian District, 100192 Beijing, China


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