Hello,Welcome:           Login line Register

line Shopping Cart line My orders line Chinese line

Cell Detection

  • TransDetect<sup>®</sup> Double-Luciferase Reporter  Assay Kit
TransDetect<sup>®</sup> Double-Luciferase Reporter  Assay Kit

TransDetect® Double-Luciferase Reporter Assay Kit


  • Product Introduction
  • Manual Download
  • References

Description

TransDetect® Double-Luciferase Reporter Assay Kit provides an efficient method to perform dual-reporter assays. In the assay, the activities of firefly (Photinus pyralis) and Renilla (sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured firstly by adding Luciferase Reaction Reagent to generate a luminescent signal. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated by adding Luciferase Reaction Reagent II to the same tube.


Storage

at -20°C in dark for one year


Kit Content


Components

FR201-01 (50 rxns)

FR201-02 (200 rxns)

Luciferase Reaction Buffer

5 ml

20 ml

Luciferase Reaction Substrate (Lyophilized)

1 vial

4 vials

Luciferase Reaction Buffer II

5 ml

20 ml

Luciferase Reaction Substrate II (50×)

100 μl

400 μl

Cell Lysis Buffer (5×)

5 ml

20 ml



Literature Journal IF Author Date Link
Activation of the SIRT1/p66shc antiapoptosis pathway via carnosic acid-induced inhibition of miR-34a protects rats against nonalcoholic fatty liver disease CELL DEATH & DISEASE 5.378 Shan W, et al. Department of Pharmacology, Dalian 2015 Jul The original link
Activation of sirtuin 1 by catalpol-induced down-regulation of microRNA-132 attenuates endoplasmic reticulum stress in colitis pharmacological research 4.48 Yongjian Xiong,et al. 2017Sep The original link
Aberrant CpG Methylation Mediates Abnormal Transcription of MAO-A Induced by Acute and Chronic L-3,4-Dihydroxyphenylalanine Administration in SH-SY5Y Neuronal Cells Neurotoxicity Research 2.9419 Zhaofei Yang,et al. 2017Apr The original link
Molecular characterization, transcriptional activity and nutritional regulation of peroxisome proliferator activated receptor gamma in Nile tilapia (Oreochromis niloticus) general and comparative endocrinology 2.667 He AY, et al. Laboratory of Aquaculture Nutrition 2015 Nov The original link

CONTACT US

Contacts:Shuyi Yan

Skype:TransGen Biotech Co., LTD

Telephone:0086-10-57815027

E-mail:info@transgenbiotech.com

Address: Building No.4, Zhongguancun Dongsheng International Science Park, 1 North Yongtaizhuang Road, Haidian District, 100192 Beijing, China


Message