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Unstained Protein Marker

  • ProteinRuler<sup>®</sup> II (12-120 kDa)
  • ProteinRuler<sup>®</sup> II (12-120 kDa)
ProteinRuler<sup>®</sup> II (12-120 kDa)ProteinRuler<sup>®</sup> II (12-120 kDa)

ProteinRuler® II (12-120 kDa)


  • Product Introduction
  • Manual Download
  • References

Description

ProteinRuler® II is composed of eight unstained recombinant proteins (20 kDa, 30 kDa, 40 kDa, 50 kDa, 60 Da, 80 kDa, 100 kDa,120 kDa) and one blue prestained recombinant protein (12 kDa). The prestained band allows monitoring electrophoresis and membrane transfer. The 50 kDa band has doubled intensity to serve as a reference band.

• MW range from 12 to 120 kDa.

• Ready-to-use format, direct load on gels without heating.


Concentration

2 μg/5 μl for 12 kDa band; 1 μg/5 μl for 50 kDa band; 0.5 μg/5 μl for each of other bands.


Storage Buffer

50 mM Tris-HCl (pH 6.8), 5 mM EDTA, 10 mM DTT, 10% glycerol, 1% SDS, 0.01% phenol red.


Storage

at -20°C for two years


Literature Journal IF Author Date Link
Efficient biodegradation of dihalogenated benzonitrile herbicides by recombinant Escherichia coli harboring nitrile hydratase-amidase pathway Biochemical Engineering Journal 2.8919 Xiaolin Pei,et al. 2017Sep The original link
Chaperones-assisted soluble expression and maturation of recombinant Co-type nitrile hydratase in Escherichia coli to avoid the need for a low induction temperature journal of biotechnology 2.667 Pei X, et al. College of Material, Chemistry and C 2015 Jun The original link
Isolation and characterization of a virulent bacteriophage SPW specific for Staphylococcus aureus isolated from bovine mastitis of lactating dairy cattle Molecular Biology Reports 2.506 Longping and Zhiying Zhang Shaanxi Key Laboratory 2014 Jul The original link
Efficient cloning and expression of a thermostable nitrile hydratase in Escherichia coli using an auto-induction fed-batch strategy Process Biochemistry 2.414 Pei X, et al. Institute of Bioengineering, Departm 2013 Sept The original link
An improved method for whole protein extraction from yeast Saccharomyces cerevisiae Yeast 1.895 Zhang T. et al. College of Animal Science and Tech 2011 Nov The original link
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