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Protein Extraction, Purification and Detection

  • ProteinExt<sup>®</sup> Mammalian Mitochondria  Isolation Kit for Cultured Cells
ProteinExt<sup>®</sup> Mammalian Mitochondria  Isolation Kit for Cultured Cells

ProteinExt® Mammalian Mitochondria Isolation Kit for Cultured Cells


  • Product Introduction
  • Manual Download
  • References

Description

ProteinExtTM Mammalian Mitochondria Isolation Kit for Cultured Cells provides a fast and efficient method to isolate mitochondria from cultured mammalian cells. This kit provides two options for the separation of mitochondria from cytosolic components: a reagent-based method or homogenization-based method. Reagent-based method uses a mild procedure to process single or multiple samples. The isolated mitochondria is suitable for a variety of downstream applications, including protein analysis, apoptosis, signal transduction and metabolic assays.


Storage

ProteinSafeTM Protease Inhibitor Cocktail and MSB at -20°C , others at 2-8°C for one year


Kit Content



Component

DE401-01 (50 rxns)

Mitochondria Isolation Buffer I (MIB I)

50 ml

Mitochondria Isolation Buffer II (MIB II)

500 μl

Mitochondria Isolation Buffer III (MIB III)

65 ml

Mitochondria Storage Buffer (MSB)

4 ml

ProteinSafeTM Protease Inhibitor Cocktail, EDTA-free (100×)

1.2 ml



Literature Journal IF Author Date Link
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