pEASY®-Blunt M3 Expression Vector utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector under regulation of enhanced CMV promoter. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.
• 5 minutes fast ligation of Pfu-amplified PCR products.
• Enhanced CMV promoter for higher protein yield.
• CMV forward primer and TK polyA reverse primer for sequencing.
• C-terminal Flag-tag for protein detection.
• Neomycin resistance gene for stable cell line selection.
• Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
• M3 Expression Plasmid included as negative control.