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Mammalian Expression Vectors

  • pEASY<sup>®</sup>-Blunt M3 Expression Kit
pEASY<sup>®</sup>-Blunt M3 Expression Kit

pEASY®-Blunt M3 Expression Kit


  • Product Introduction
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Description

pEASY®-Blunt M3 Expression Vector utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector under regulation of enhanced CMV promoter. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.

• 5 minutes fast ligation of Pfu-amplified PCR products.

• Enhanced CMV promoter for higher protein yield.

• CMV forward primer and TK polyA reverse primer for sequencing.

• C-terminal Flag-tag for protein detection.

• Neomycin resistance gene for stable cell line selection.

Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

• M3 Expression Plasmid included as negative control.


Storage

Competent Cell at -70°C for six months; others at -20°C for nine months


Kit Content


Component

CM311-01 (10 rxns)

pEASY ®-Blunt M3 Expression Vector (15 ng/μl)

10 µl

M3 Expression Plasmid (Negative Control) (15 ng/μl)

10 µl

MControl Template (5 ng/μl)

10 µl

MControl Forward Primers (10 μM)

10 µl

MControl Reverse Primers (10 μM)

10 µl

CMV Forward Primer (10 μM)

50 µl

TK PolyA Reverse Primer (10 μM)

50 µl

Trans1-T1 Phage Resistant

5×100 μl

Chemically Competent Cell



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