Hello,Welcome:           Login line Register

line Shopping Cart line My orders line Chinese line

Expression Competent Cells

  • TransB(DE3) Chemically Competent Cell
  • TransB(DE3) Chemically Competent Cell
TransB(DE3) Chemically Competent CellTransB(DE3) Chemically Competent Cell

TransB(DE3) Chemically Competent Cell


  • Product Introduction
  • Manual Download
  • References

Description

BL21(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).


Genotype

F- ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (KanR,TetR)


Features

• Transformation efficiency: >107 cfu/μg (pUC19 DNA).

• KanR and TetR.

• Thioredoxin reductase (trxB) and glutathione reductase (gor) mutation greatly facilitates cytoplasmic disulfide bond formation.

• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.


Storage

at -70°C for six months. Do not store in liquid nitrogen

Literature Journal IF Author Date Link
Biochemical characteristics of New Delhi metallo-β-lactamase-1 show unexpected difference to other MBLs. PLoS One 3.73 T Li, et al. State Key Laboratory of Pathogens and 2013 Apr The original link
Expression and Characterization of a Recombinant Laccase with Alkalistable and Thermostable Properties from Streptomyces griseorubens JSD-1 Applied Biochemistry and Biotechnology 1.735 Haiwei Feng, et al. 2015 Mar The original link
The lytic activity of recombinant phage lysin LysKΔamidase against staphylococcal strains associated with bovine and human infections in the Jiangsu province of China research in veterinary science 1.2979 Yan Zhou,et al. 2017Apr The original link
Message