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Expression Competent Cells

  • BL21 (DE3) pLysS Chemically  Competent Cell
BL21 (DE3) pLysS Chemically  Competent Cell

BL21 (DE3) pLysS Chemically Competent Cell

  • Product Introduction
  • Manual Download
  • References


BL21(DE3) pLysS Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to chloramphenicol(CamR) and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).


F- ompT hsdS(rB-mB-)gal dcm(DE3)pLysS Camr


• Transformation efficiency: >107 cfu/μg (pUC19 DNA).

• CamR.

• Contains pLysS plasmid that expresses the T7 lysozyme gene to reduce the background of the target protein expression without

disturbing IPTG functioning.

• Suitable for non-toxic and toxic protein’s expression.

• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.


at -70°C for six months. Do not store in liquid nitrogen

Literature Journal IF Author Date Link
A single fluorescent protein-based sensor for in vivo 2-oxogluatarate detection in cell Biosensors and Bioelectronics 5.437 Chang Zhang, et al. Lab of Biosystems and Microana 2014 Apr The original link
A Dimerization-Dependent Mechanism Drives the Endoribonuclease Function of PRRSV nsp11 J Virol. 4.439 Yuejun Shi, et al. 2016 Feb The original link
Quantitative monitoring of 2-oxoglutarate in Escherichia coli cells by a fluorescence resonance energy transfer-based biosensor Appl Microbiol Biotechnol 3.689 Chang Zhang, et al. Laboratory of Biosystems and M 2013 Jul The original link
Amino acid residue Y196E substitution and C-terminal peptide synergistically alleviate the toxicity of Clostridium perfringens epsilon toxin Toxicon 2.492 Wenwu Yao, et al. 2015 Apr The original link