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Expression Competent Cells

  • BL21(DE3) Chemically Competent Cell
BL21(DE3) Chemically Competent Cell

BL21(DE3) Chemically Competent Cell

  • Product Introduction
  • Manual Download
  • References


BL21(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).


F- ompT hsdS(rB-mB-)gal dcm(DE3)


• Transformation efficiency: >107 cfu/μg (pUC19 DNA).

• DE3 strains contains the λDE3 lysogen that carries the gene for T7 RNA polymerase.

• Suitable for T7 and T7lac such as pET, pEASY®.

• Suitable for high expression of non-toxic protein.

• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.


at -70°C for six months. Do not store in liquid nitrogen

Literature Journal IF Author Date Link
A genetically encoded biosensor for in vitro and in vivo detection of NADP+ Biosensors and Bioelectronics 6.409 Feng-Lan Zhao, et al. 2015 Oct The original link
Utilization of biodiesel by-product as substrate for high-production of ??-farnesene via relatively balanced mevalonate pathway in Escherichia coli Bioresource Technology 5.65 Shengping You et al Chemical Engineering Research 2017 Jun The original link
General Bioluminescence Resonance Energy Transfer Homogeneous Immunoassay for Small Molecules Based on Quantum Dots Anal. Chem. 5.636 Xuezhi Yu, et al. 2016 Mar The original link
Functional Analysis of Esterase TCE2 Gene from Tetranychus cinnabarinus (Boisduval) involved in Acaricide Resistance Sci Rep. 5.578 Li Shi, et al. 2016 Jan The original link
The antitumor activity and preliminary modeling on the potential mechanism of action of human peroxiredoxin-5 Oncotarget 5.0941 Juanjuan Liu,et al. 2017Mar The original link