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Prokaryotic Expression Vectors

  • pEASY<sup>®</sup>-Blunt E2 Expression Kit
pEASY<sup>®</sup>-Blunt E2 Expression Kit

pEASY®-Blunt E2 Expression Kit


  • Product Introduction
  • Manual Download
  • References

Description

pEASY®-Blunt E2 Expression Vector is constructed from pET vector. It utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.

• 5 minutes fast ligation of Pfu-amplified PCR products.

• Ampicillin resistance.

• T7 promoter primer and T7 terminator primer for sequencing.

• Bacteriophage T7lac promoter for high level expression.

• C-terminal 6×His tag for easy purification.

• Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

• E2 Expression Plasmid included as negative control.


Storage

Chemically Competent Cell at -70°C for six months; others at -20°C for nine months


Kit Content


Component

CE211-01 (10 rxns)

pEASY®-Blunt E2 Expression Vector (15 ng/μl)

10 µl

E2 Expression Plasmid (Negative Control) (15 ng/μl)

10 µl

EControl Template (5 ng/μl)

10 µl

EControl Forward Primer (10 μM)

10 µl

EControl Reverse Primer (10 μM)

10 µl

T7 Promoter Primer (10 μM)

50 µl

T7 Terminator Primer (10 μM)

50 µl

Trans1-T1 Phage Resistant Chemically Competent Cell

5×100 μl



Literature Journal IF Author Date Link
A Novel NHERF1 Mutation in Human Breast Cancer and Effects on Malignant Progression. anticancer research 1.9 Xiaomei Yang Shan Cheng, Department of Biochemist 2017 Jan The original link
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