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Prokaryotic Expression Vectors

  • pEASY<sup>®</sup>-Blunt E1 Expression Kit
pEASY<sup>®</sup>-Blunt E1 Expression Kit

pEASY®-Blunt E1 Expression Kit


  • Product Introduction
  • Manual Download
  • References

Description

pEASY®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.

• 5 minutes fast ligation of Pfu-amplified PCR products.

• Ampicillin resistance.

• T7 promoter primer and T7 terminator primer for sequencing.

• Bacteriophage T7lac promoter for high level expression.

• N-terminal 6×His tag for easy purification.

• Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

• E1 Expression Plasmid included as negative control.


Storage

Competent Cell at -70°C for six months; others at -20°C for nine months


Kit Content


Component

CE111-01 (10 rxns)

pEASY®-Blunt E1 Expression Vector (15 ng/μl)

10 µl

E1 Expression Plasmid (Negative Control) (15 ng/μl)

10 µl

EControl Template (5 ng/μl)

10 µl

EControl Forward Primer (10 μM)

10 µl

EControl Reverse Primer (10 μM)

10 µl

T7 Promoter Primer (10 μM)

50 µl

T7 Terminator Primer (10 μM)

50 µl

Trans1-T1 Phage Resistant Chemically Competent Cell

5×100 μl



Literature Journal IF Author Date Link
A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in Plants Plant Physiology 7.367 Dongyan Cao, et al. College of Food Science and Nu 2017 Apr The original link
Identification of a novel class B scavenger receptor homologue in Portunus trituberculatus: Molecular cloning and microbial ligand binding fish & shellfish immunology 3.025 Yang N, et al. Key Laboratory of the Ministry of E 2016 Nov The original link
Genome-wide identification and functional analysis of S-RNase involved in the self-incompatibility of citrus molecular genetics and genomics 2.622 Liang M, et al. Key Laboratory of Horticultural Pl 2016 Dec The original link
Functional characterization of aphid OR insect science 2.026 Rui-Bin Zhang,et al. 2017Jul The original link
Characterization of a neutral recombinant xylanase from Thermoactinospora rubra YIM 77501T antonie van leeuwenhoek international journal of g 1.944 Yin YR, et al. Yunnan Institute of Microbiology, Y 2017 Mar The original link
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