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Next Generation Sequencing

  • TransNGS<sup>TM</sup> Library Quantification Kit for Illumina®
  • TransNGS<sup>TM</sup> Library Quantification Kit for Illumina®
TransNGS<sup>TM</sup> Library Quantification Kit for Illumina®TransNGS<sup>TM</sup> Library Quantification Kit for Illumina®

TransNGSTM Library Quantification Kit for Illumina®


  • Product Introduction
  • Manual Download
  • References

Description

TransNGSTM Library Quantification Kit for Illumina® provides all reagents needed for qPCR based quantification of DNA libraries prepared for Illumina next generation sequencing platforms. Five linear DNA Standards with different GC contents are provided for customers to choose from. The kit also contains PCR primers which target the P5 and P7 adaptor sequences and TransNGSTM Library Quantification qPCR SuperMix (2×) for library amplifications.

Hightlights

Five sets of linear DNA Standards (25%, 37.5%, 50%, 62.5%, and 75% of GC contents) to choose from Each DNA Standard contains six 10-fold dilution series of a linear 420 bp template TransNGSTM Library Quantification qPCR SuperMix (2×) enables accurate library amplification with various GC contents.

Storage

at -20°C in dark for one year

Kit Content

Component

KQ101-01 (100 rxns)

KQ101-02 (500 rxns)

TransNGSTM Library Quantification qPCR SuperMix (2×)

1 ml

5×1 ml

Library Quantification Primer Mix (20×)

100 μl

500 μl

DNA Standards (S1-S6)

24 μl each

120 μl each

Library Dilution Buffer (10×)

1 ml

5 ml

Passive Reference Dye (50×)

40 μl

200 μl

ddH2O

10 ml

50 ml

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