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RNA Purification

  • EasyPure<sup>®</sup> Viral DNA/RNA Kit
EasyPure<sup>®</sup> Viral DNA/RNA Kit

EasyPure® Viral DNA/RNA Kit


  • Product Introduction
  • Manual Download
  • References

Description

EasyPure Viral DNA/RNA Kit provides a simple and fast column based method to isolate viral DNA/RNA from up to 200 of plasma, serum, body fluid and mammalian cell supernatant. Samples are lysed with unique lysis buffer and DNA/RNA is enriched by carrier RNA. DNA/RNA is bound to silica membrane. After washing, high quality DNA/RNA is eluted from the column.


Applications

PCR, RT-PCR, qPCR and qRT-PCR


Storage

Carrier RNA and Proteinase K solution at -20°C for one year; others at room temperature for one year


Kit Content


Component

ER201-01 (50 rxns)

Binding Buffer 5 (BB5)

15 ml

Wash Buffer 5 (WB5)

12 ml

Proteinase K (20 mg/ml)

1 ml

Carrier RNA (1 μg/μl)

310 μl

RNase-free Water

10 ml

RNase-free Tube (1.5 ml)

50 each

RNA Spin Columns with Collection Tubes

50 each



Literature Journal IF Author Date Link
A Novel Enterovirus 71 (EV71) Virulence Determinant: The 69th Residue of 3C Protease Modulates Pathogenicity Front Cell Infect Microbiol 5.2179 Bingqing Li,et al. 2017Feb The original link
Genetic Diversity and Positive Selection Analysis of Classical Swine Fever Virus Envelope Protein Gene E2 in East China under C-Strain Vaccination FRONTIERS IN MICROBIOLOGY 4.074 Dongfang Hu,et al. 2016Feb The original link
Serum HBV DNA, RNA and HBsAg: which correlated better to intrahepatic covalently 2closed circular DNA before and after nucleos(t)ide analogue treatment? journal of clinical microbiology 3.993 Yuhua Gao,et al. 2017Jul The original link
Clinical evaluation of a new single-tube multiplex reverse transcription PCR assay for simultaneous detection of eleven respiratory viruses, Mycoplasma pneumoniae and Chlamydia in hospitalized children with acute respiratory infections diagnostic microbiology and infectious disease 2.401 Meng-chuan Zhao,et al. 2017Jun The original link
A loop-mediated isothermal amplification assay for the visual detection of duck circovirus Virology Journal 2.092 Liji Xie, et al. Department of Biotechnology, Gua 2014 Apr The original link
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