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Plasmid DNA Purification and E. Coli Medium

  • EasyPure<sup>®</sup> HiPure Plasmid MiniPrep Kit
EasyPure<sup>®</sup> HiPure Plasmid MiniPrep Kit

EasyPure® HiPure Plasmid MiniPrep Kit


  • Product Introduction
  • Manual Download
  • References

Description

EasyPure® HiPure Plasmid MiniPrep Kit provides an efficient way to isolate high yield (up to 40 μg) and high quality plasmid DNA from ≤20 ml (LB) or ≤4 ml (ArtMedia® Plasmid Culture) of bacterial culture. Endotoxin is removed by a simple incubation on column with a novel buffer. The purified plasmid DNA is suitable for a variety of down stream molecular biology applications, including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection.

• Fast: the whole procedure can be performed in 20 minutes.

• Simple: endotoxin is removed on column.

• High yield: DNA yield up to 40 μg.


Storage

RNase A at -20°C for one year; others at room temperature (15-25°C) for one year


Kit Content


Component

EM111-01 (50 rxns)

Resuspension Buffer (RB)

15 ml

Lysis Buffer (LB)

15 ml

Neutralization Buffer (NB)

20 ml

ToxinOut Buffer (TB)

15 ml

Wash Buffer (WB)

10 ml

Elution Buffer (EB)

5 ml

RNase A (10 mg/ml)

150 μl

Mini-Plasmid Spin Columns with Collection Tubes

50 each



Literature Journal IF Author Date Link
Transcriptional Regulation of Human UDP-glucuronosyltransferase 2B10 by Farnesoid X Receptor in Human Hepatoma HepG2 Cells Molecular Pharmaceutics 4.44 Danyi Lu,et al. 2017Mar The original link
Production and evolution of a ScFv antibody for immunoassay of residual phenothiazine drugs in meat based on computational simulation Analytical Methods 1.8999 Fang Shu Shi, et al. 2017Apr The original link
Blueberries inhibit cyclooxygenase-1 and cyclooxygenase-2 activity in human epithelial ovarian cancer ONCOLOGY LETTERS 1.3899 WUMEI LIN,et al. 2017Apr The original link
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