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Genomic DNA Purification

  • EasyPure<sup>®</sup> Bacteria Genomic DNA Kit
EasyPure<sup>®</sup> Bacteria Genomic DNA Kit

EasyPure® Bacteria Genomic DNA Kit

  • Product Introduction
  • Manual Download
  • References


EasyPure® Bacteria Genomic DNA Kit uses lysozyme and moderate lysis buffer to lyse cells. Proteinase K is used for protein digestion and RNase A used for RNA digestion. DNA is specifically bound to silica-based column in hypersaline condition, and DNA is eluted by low salt and high pH solution. This kit is suitable for isolating high quality genomic DNA from Gram-positive and Gram-negative bacteria. The isolated DNA is suitable for PCR, restriction enzyme digestion, and Southern blot.

• Fast: the whole process can be completed in 50 minutes.

• High yield: DNA yield up to 20 μg


PCR, restriction enzyme digestion, and Southern blot.


RNase A and Proteinase K solutions at -20°C for one year; others at room temperature for one year

Kit Content


EE161-01 (50 rxns)

EE161-11 (50 rxns)

Resuspension Buffer11 (RB11)

12 ml

Lysis Buffer11 (LB11)

6 ml

Binding Buffer11 (BB11)

10 ml

Clean Buffer 11 (CB11)

55 ml

Wash Buffer 11 (WB11)

12 ml

Elution Buffer (EB)

25 ml

RNase A (10 mg/ml)

1 ml (EE161-01)

0 (EE161-11)

Proteinase K (20 mg/ml)

1 ml

Genomic Spin Columns with Collection Tubes

50 each

Sample requirement

Gram-positive or Gram-negative bacteria cells ≤109

Literature Journal IF Author Date Link
Biochemical characterization of a novel -galactosidase from Paenibacillus barengoltzii suitable for lactose hydrolysis and galactooligosaccharides synthesis International Journal of Biological Macromolecules 3.671 Yu Liu, et al. 2017 Nov The original link
Biochemical characterization of a novel L-Asparaginase from Paenibacillus barengoltzii being suitable for acrylamide reduction in potato chips and mooncakes international journal of biological macromolecules 3.671 Ran Shi,et al. 2017Mar The original link
Phaeocystidibacter marisrubri sp. nov., a member of the family Cryomorphaceae isolated from Red Sea sediment Int J Syst Evol Microbiol 2.511 Xiaowei Zheng, et al. 2015 Jul The original link
Determination of the microbial community features of Haemaphysalis flava in different developmental stages by high-throughput sequencing journal of basic microbiology 1.4379 Deyong Duan,et al. 2017Apr The original link
PCR denaturing gradient gel electrophoresis as a useful method to identify of intestinal bacteria flora in Haemaphysalis flava ticks acta parasitologica 1.1599 Tian-Yin Cheng,et al. 2017Jun The original link