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Genomic DNA Purification

  • EasyPure<sup>®</sup> Blood Genomic DNA Kit
  • EasyPure<sup>®</sup> Blood Genomic DNA Kit
EasyPure<sup>®</sup> Blood Genomic DNA KitEasyPure<sup>®</sup> Blood Genomic DNA Kit

EasyPure® Blood Genomic DNA Kit


  • Product Introduction
  • Manual Download
  • References

Description

EasyPure® Blood Genomic DNA Kit provides a simple and convenient way to isolate high quality genomic DNA from 5-250 μl of fresh or frozen blood. Whole blood is incubated with binding/lysis buffer to release DNA. DNA is bound to silica-based column.

• Simple and fast, red cell lysis buffer is no longer needed.

• Complete removal of contaminants and inhibitors.

• DNA yield up to 40 μg.

• Column based purification, no organic extraction or ethanol precipitation.

• Suitable for EDTA, sodium citrate and heparin-anticoagulated fresh or frozen blood in a volume of 5 to 250 μl.


Applications

PCR, restriction enzyme digestion and Southern blot.


Stoarge

RNase A and Proteinase K solutions at -20°C for one year; others at room temperature for one year


Kit Content


Component

EE121-01 (50 rxns)

EE121-02 (200 rxns)

EE121-11 (50 rxns)

EE121-12 (200 rxns)

Binding Buffer 3 (BB3)

30 ml

110 ml

Clean Buffer 3 (CB3)

6 ml

24 ml

Wash Buffer 3 (WB3)

12 ml

2×22 ml

Elution Buffer (EB)

25ml

80 ml

RNase A (20 mg/ml)

500 μl (EE121-01)

2×1 ml (EE121-02)

0 (EE121-11)

0 (EE121-12)

Proteinase K (20 mg/ml)

1 ml

4×1 ml

Genomic Spin Columns with Collection Tubes

50 each

200 each




Literature Journal IF Author Date Link
Assessment of genetic diversity in Chinese eared pheasant using fluorescent-AFLP markers molecular phylogenetics and evolution 3.792 Li X,et al. College of Veterinary Medicine, China 2010 Nov The original link
Association of polymorphisms in PATE1 gene with idiopathic asthenozoospermia in Sichuan, China ournal of reproductive immunology 3.202 Zhang S, et al. Key Laboratory of Bio-Resources an 2016 Nov The original link
Genetic diversities of cytochrome B in Xinjiang Uyghur unveiled its origin and migration history BMC Genetics 2.808 A Ablimit. et al. Life science and technology, Xin 2013 Oct The original link
Increased plasma orexin-A levels in patients with insomnia disorder are not associated with prepro-orexin or orexin receptor gene polymorphisms peptides 2.778 Shi Tang,et al. 2017Feb The original link
Genetic variations in inflammatory response genes and their association with the risk of prostate cancer BIOMED RESEARCH INTERNATIONAL 2.134 Cui X, et al. Department of Urology, Xuanwu Hospit 2015 The original link
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