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Mutagenesis System

  • Fast Mutagenesis System
Fast Mutagenesis System

Fast Mutagenesis System

  • Product Introduction
  • Manual Download
  • References


• Primers anneal to the DNA template, mutant strands are synthesized with 2×TransStart® FastPfu PCR SuperMix.

• In vitro digestion of non-mutated parental plasmid (methylated plasmid) with DMT enzyme and in vivo degradation of non-mutated parental plasmid (methylated plasmid) with DMT Chemically Competent Cell, so as to efficiently select mutant clones.


• Mutation sites on both primers to improve mutation efficiency.

• Partially overlapping primers for exponential DNA amplification.

• Fast (4 kb/min) and high fidelity ( 54-fold fidelity as compared to EasyTaq® DNA Polymerase) 2xTransStart® FastPfu PCR SuperMix for DNA amplification.

• Double digestions (in vitro and in vivo) of parental plasmids to enhance mutation efficiency.


Competent Cell at -70°C for six months; others at -20°C for two years

Kit Content


FM111-01 (10 rxns)

FM111-02 (20 rxns)

TransStart® FastPfu PCR SuperMix

250 µl

500 µl

DMT Enzyme (10 units/μl)

10 µl

20 µl

DMT Chemically Competent Cell

10×50 µl

20×50 µl


1 ml

1 ml

SControl Plasmid (5 ng/µl)

10 µl

20 µl

SControl Primers (10 µM )

10 µl

20 µl

Literature Journal IF Author Date Link
Whole-genome sequencing of the snub-nosed monkey provides insights into folivory and evolutionary history. Nature genetics 29.648 Xuming Zhou, et al. 2014 Dec The original link
Crystal structure of PXY-TDIF complex reveals a conserved recognition mechanism among CLE peptide-receptor pairs Cell Research 14.812 Heqiao Zhang, et al. Ministry of Education Key Lab 2016 jan The original link
Maternal xNorrin, a canonical Wnt signaling agonist and TGF-β antagonist, controls early neuroectoderm specification in Xenopus PloS Biology 12.69 Xu S, et al. State Key Laboratory of Molecular Dev 2012 The original link
PGE2 Promotes Hepatic Biliary Cholesterol Excretion by EP3-mediated HNF4α/CYP7A1 Pathway in Mice hepatology 11.711 Shuai Yan,et al. 2016Dec The original link
Plastid-nucleus communication involves calcium-modulated MAPK signalling Nature communications 11.329 Hailong Guo, et al. Photosynthesis Research Center 2016 jun The original link