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Cloning Competent Cells

  • DMT Chemically Competent Cell
  • DMT Chemically Competent Cell
DMT Chemically Competent CellDMT Chemically Competent Cell

DMT Chemically Competent Cell

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F- φ80 lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK-, mK+) phoA supE44 thi-1 gyrA96relA1 tonA


• High transformation efficiency: >108 cfu/μg (pUC19 DNA).

• Resistance to T1 and T5 phage.

• In vivo digestion of methylated DNA, suitable for site-directed mutagenesis.


at -70 °C for six months


Higher efficiency transformation can be achieved by transforming cells immediately following thawing.

Avoid repeated thawing.

Gentle handling is required for the entire procedure.

Literature Journal IF Author Date Link
Characterization of EndoTT, a novel single-stranded DNA-specific endonuclease from Thermoanaerobacter tengcongensis nucleic acids research 9.202 Zhang S, et al. State Key Laboratory of Microbial 2010 Jun The original link
Improving the thermostability of a fungal GH11 xylanase via site-directed mutagenesis guided by sequence and structural analysis Biotechnology for Biofuels 6.4439 Nanyu Han,et al. 2017 May The original link
A Novel Enterovirus 71 (EV71) Virulence Determinant: The 69th Residue of 3C Protease Modulates Pathogenicity Front Cell Infect Microbiol 5.2179 Bingqing Li,et al. 2017Feb The original link
The N-Terminal GH10 Domain of a Multimodular Protein from Caldicellulosiruptor bescii Is a Versatile Xylanase/β-Glucanase That Can Degrade Crystalline Cellulose Applied and Environmental Microbiology 3.668 Xianli Xue, et al. 2015 Mar The original link