Hello,Welcome:           Login line Register

line Shopping Cart line My orders line Chinese line

Cloning Competent Cells

  • Trans2-Blue Chemically Competent Cell
  • Trans2-Blue Chemically Competent Cell
Trans2-Blue Chemically Competent CellTrans2-Blue Chemically Competent Cell

Trans2-Blue Chemically Competent Cell


  • Product Introduction
  • Manual Download
  • References

Description

Trans2-Blue Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 109 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to tetracycline (TetR) and chloramphenicol (CamR).


Genotype

TetRΔ(mcrA)183Hte[F′{proAB lacIq lacZΔM15Tn10(TetR)AmyCamR}]Δ(mcrCB-hsdSMR-mrr)173 endA1supE44 thi-1 recA1 gyrA96 relA1


Features

• High transformation efficiency (>1×109cfu/μg DNA).

• Suitable for larger plasmids and recombinant products.

• Reduced preference for plasmid size, suitable for library construction.

• Used in Blue/White selection.


Storage

at -70 °C for six months


Notes

Higher efficiency transformation can be achieved by transforming cells immediately following thawing.

Avoid repeated thawing.

Gentle handling is required for the entire procedure


Literature Journal IF Author Date Link
Identification of genes directly involved in shell formation and their functions in pearl oyster, Pinctada fucata PLoS One 3.5328 Fang D, et al. Institute of Marine Biotechnology, 2011 The original link
Characterization of an insect heterodimeric voltage-gated sodium channel with unique alternative splicing mode comparative biochemistry and physiology b-biochemi 1.651 Jiang XZ, et al. Key Laboratory of Entomology and 2017 Jan The original link
Message