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Cloning Competent Cells

  • Trans1-Blue Chemically Competent Cell
  • Trans1-Blue Chemically Competent Cell
Trans1-Blue Chemically Competent CellTrans1-Blue Chemically Competent Cell

Trans1-Blue Chemically Competent Cell


  • Product Introduction
  • Manual Download
  • References

Description

Trans1-Blue Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to tetracycline (TetR).


Genotype

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 (rk-,mk+), relA1 lac [F' proAB lacIqZΔM15: Tn10 (TetR)]


Features

• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).

• TetR.

• Blue/white selection.


Storage

at -70 °C for six months


Notes

Higher efficiency transformation can be achieved by transforming cells immediately following thawing.

Avoid repeated thawing.

Gentle handling is required for the entire procedure.



Literature Journal IF Author Date Link
The N-Terminal GH10 Domain of a Multimodular Protein from Caldicellulosiruptor bescii Is a Versatile Xylanase/β-Glucanase That Can Degrade Crystalline Cellulose Applied and Environmental Microbiology 3.668 Xianli Xue, et al. 2015 Mar The original link
Controlling microbial PHB synthesis via CRISPRi applied microbiology and biotechnology 3.42 Dan Li,et al. 2017May The original link
Molecular cloning and characterization of DXS and DXR genes in the terpenoid biosynthetic pathway of Tripterygium wilfordii international journal of molecular sciences 3.257 Tong Y, et al. School of Traditional Chinese Medic 2015 Oct The original link
Metabolic engineering of an E. coli ndh knockout strain for PHB production from mixed glucose-xylose feedstock journal of chemical technology and biotechnology 3.1349 Guangxin Huo,et al. 2017May The original link
Engineering Escherichia coli for poly-(3-hydroxybutyrate) production guided by genome-scale metabolic network analysis enzyme and microbial technology 2.502 Yangyang Zheng,et al. 2017Nov The original link
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