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Cloning Competent Cells

  • Trans110 Chemically Competent Cell
  • Trans110 Chemically Competent Cell
Trans110 Chemically Competent CellTrans110 Chemically Competent Cell

Trans110 Chemically Competent Cell


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Description

Trans110 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 106 cfu/μg DNA (tested by pUC19 plasmid DNA).


Genotype

rpsL (StrR) thr leu thi-1 lacY galK galT ara tonA tsx dam dcm supE44 Δ(lac-proAB) /F′ [traD36proAB lacIq lacZΔM15]


Features

• High transformation efficiency: >106 cfu/μg ( pUC19 DNA).

• Unmethylated DNA due to dam-/dcm-.

• StrR.


Storage

at -70 °C for six months


Notes

Higher efficiency transformation can be achieved by transforming cells immediately following thawing.

Avoid repeated thawing.

Gentle handling is required for the entire procedure.

Literature Journal IF Author Date Link
MAPK signaling pathway alters expression of midgut ALP and ABCC genes and causes resistance to Bacillus thuringiensis Cry1Ac toxin in diamondback moth PLoS Genetics 8.167 Zhaojiang Guo?? et al 2015 Apr The original link
Bacillus thuringiensis subsp. sichuansis strain MC28 produces a novel crystal protein with activity against Culex quinquefasciatus larvae World Journal of Microbiology and Biotechnology 1.262 Peng Guan, et al. State Key Laboratory of Hybrid R 2014 Apr The original link
Cloning and characterisation of a novel cry1H gene effective against lepidopterous larvae from a Bacillus thuringiensis strain Biocontrol Science and Technology 0.848 Yu Z, et al. Sichuan agricultural university 2015 The original link
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