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Cloning Vectors

  • pEASY<sup>®</sup>-Blunt Simple Cloning Kit
pEASY<sup>®</sup>-Blunt Simple Cloning Kit

pEASY®-Blunt Simple Cloning Kit


  • Product Introduction
  • Manual Download
  • References

Description

pEASY ®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY ® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products.

•5 minutes fast ligation of Pfu-amplified PCR products.

•Kanamycin and Ampicillin resistance genes for selection.

•Easy blue/white selection.

•SR primer and M13 forward primer for sequencing.

•T7 promoter for in vitro transcription.

Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage

Competent Cell at -70°C for six months; others at -20°C for one year

Kit Content

Component

CB111-01

CB111-02

(20 rxns)

(60 rxns)

pEASY ®-Blunt Simple Cloning Vector (10 ng/μl)

20µl

3×20µl

Control Template (5 ng/μl)

5µl

5µl

Control Primers (10 μM)

5µl

5µl

M13 Forward Primer (10 μM)

50µl

150µl

M13 Reverse Primer (10 μM)

50µl

150µl

SR Primer (10 μM)

50µl

150µl

Trans1-T1 Phage Resistant Chemically Competent Cell

10×100µl

30×100µl




Literature Journal IF Author Date Link
Phylogenetically diverse endozoic fungi in the South China Sea sponges and their potential in synthesizing bioactive natural products suggested by PKS gene and cytotoxic activity analysis Fungal Diversity 5.319 Yu Z, et al. Marine Biotechnology Laboratory, Stat 2013 Jan The original link
Two Novel Fungal Phenolic UDP Glycosyltransferases from Absidia coerulea and Rhizopus japonicus applied and environmental microbiology 3.8229 Kebo Xie,et al. 2017Sep The original link
RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid Organogenesis PLOS ONE 3.73 Bin Gao,et al. 2013 Aug The original link
Polymorphisms of Chicken TLR3 and 7 in Different Breeds PLOS ONE 3.73 Wenke Ruan 2015 Mar The original link
Molecular cloning and functional characterization of multiple NADPH-cytochrome P450 reductases from Andrographis paniculata. international journal of biological macromolecules 3.6 Huixin Lin et al National Resource Center for Chin 2017 Apr The original link
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