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Cloning Vectors

  • pEASY<sup>®</sup>-Blunt Cloning Kit
pEASY<sup>®</sup>-Blunt Cloning Kit

pEASY®-Blunt Cloning Kit


  • Product Introduction
  • Manual Download
  • References

Description

pEASY ®-Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products.

•5 minutes fast ligation of Pfu-amplified PCR products.

•Kanamycin and Ampicillin resistance genes for selection.

•Easy blue/white selection.

•T7 promoter, M13 forward and M13 reverse primers for sequencing.

•T7 promoter for in vitro transcription.

Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage

Competent Cell at -70°C for six months; others at -20°C for 9 months

Kit Content

Component

CB101-01

CB101-02

(20 rxns)

(60 rxns)

pEASY ®- Blunt Cloning Vector (10 ng/μl)

20µl

3×20 µl

Control Template (5 ng/μl)

5µl

5µl

Control Primers (10 μM)

5µl

5µl

M13 Forward Primer (10 μM)

50µl

150µl

M13 Reverse Primer (10 μM)

50µl

150µl

Trans1-T1 Phage Resistant

10×100 μl

30×100μl

Chemically Competent Cell



Literature Journal IF Author Date Link
Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew Nature Biotechnology 39.08 Yanpeng Wang, et al. 2014 Jul The original link
D14–SCFD3-dependent degradation of D53 regulates strigolactone signaling Nature 38.597 Feng Zhou,et al. 2013 Dec The original link
"Perfect" designer chromosome V and behavior of a ring derivative Science 34.661 Xie Z, et al. Key Laboratory of Systems Bioenginee 2017 Mar The original link
Targeted genome modification of crop plants using a CRISPR-Cas system Nature Biotechnology 32.44 Qiwei Shan,et al. 2013 Aug The original link
Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds Cell Research 12.413 Junqing Ye, et al. 2015 Dec The original link
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