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Fast Restriction Enzyme

  • FlyCut<sup>®</sup> SpeI
FlyCut<sup>®</sup> SpeI

FlyCut® SpeI


  • Product Introduction
  • Manual Download
  • References

Description

FlyCut® SpeI is expressed and purified from E.coli that carries the recombinant SpeI gene. The molecular weight is

21.7 kDa, with the recognition site at A^CTAGT. The reaction is conducted for 5-15 minutes at 37oC, and heat-inactivated at 80oC for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.


Concentration

20,000 units/ml


Recognition Site

5’...ACTAGT...3’

3’...TGATCA...5’


Applications

Genomic DNA, plasmid DNA, PCR product


Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.


Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol


10×FlyCut® Buffer

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml


Storage

at -20℃ for two years


Kit Content


Component

JS601-01

JS602-02

FlyCut® SpeI

250 units

2×250 units

10×FlyCut® Buffer

250 μl

250 μl

10×DNA Loading Buffer

1 ml

1 ml


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