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Fast Restriction Enzyme

  • FlyCut<sup>®</sup> SacI
FlyCut<sup>®</sup> SacI

FlyCut® SacI


  • Product Introduction
  • Manual Download
  • References

Description

 

 

FlyCut® SacI is expressed and purified from E.coli that carries the recombinant SacI gene. The molecular weight is 40.8 kDa, with the recognition site at GAGCT^C. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 80oC for 20 minutes.This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Concentration

 

20,000 units/ml

Recognition Site

 

 

5’...GAGCT↓C...3’

3’...C↑TCGAG...5’

Applications

 

Genomic DNA, plasmid DNA, PCR product

Unit Definition

 

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

 

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol

10×FlyCut® Buffer

 

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

Storage

 

at -20℃ for two years

Kit Content

 

 

Component

JS101-01

JS101-02

FlyCut® SacI

1,000 units

2×1,000 units

10×FlyCut® Buffer

250 μl

500 μl

10×DNA Loading Buffer

1 ml

1 ml


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