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Fast Restriction Enzyme

  • FlyCut<sup>®</sup> NdeI
FlyCut<sup>®</sup> NdeI

FlyCut® NdeI


  • Product Introduction
  • Manual Download
  • References

Description

FlyCut® NdeI is expressed and purified from E.coli that carries the recombinant NdeI gene. The molecular weight is 46.5 kDa, with the recognition site at CA^TATG. The reaction is conducted for 5-10 minutes at 37, and heat-inactivated at 65 for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.


Concentration

20,000 units/ml


Recognition Site

5’...CA↓TATG...3’

3’...GTAT↑AC...5’


Applications

Genomic DNA, plasmid DNA, PCR product


Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.


Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol


10×FlyCut® Buffer


500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

Storage

at -20℃ for two years


Kit Contents



Component

JN201-01

JN201-02

FlyCut® NdeI

2,000 units

2×2,000 units

10×FlyCut® Buffer

500 μl

1 ml

10×DNA Loading Buffer

1 ml

1 ml



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