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Fast Restriction Enzyme

  • FlyCut<sup>®</sup> BsgI
FlyCut<sup>®</sup> BsgI

FlyCut® BsgI

  • Product Introduction
  • Manual Download
  • References


FlyCut® BsgI is expressed and purified from E.coli that carries the recombinant BsgI gene. The molecular weight is 122 kDa, with the recognition site at GTGCAG(N)14∨(N)2 . The reaction is conducted for 5-15 minutes at 37, and heat-inactivated at 65 for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.


5,000 units/ml

Recognition Site

5’...GTGCAG(N)16 ...3’

3’...CACGTC(N)14 ...5’


Genomic DNA, plasmid DNA, PCR product

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol

10×FlyCut® Buffer

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml


at -20℃ for two years

Kit Contents




FlyCut® BsgI

50 units

2×50 units

10×FlyCut® Buffer

1 ml

1 ml

10×DNA Loading Buffer

1 ml

1 ml

Literature Journal IF Author Date Link