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Fast Restriction Enzyme

  • FlyCut<sup>®</sup> BamHI
FlyCut<sup>®</sup> BamHI

FlyCut® BamHI

  • Product Introduction
  • Manual Download
  • References


FlyCut® BamHI is expressed and purified from E.coli that carries the recombinant BamHI gene. The molecular weight is 27.5 kDa, with the recognition site at G^GATCC. The reaction is conducted for 5-10 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site




Genomic DNA, plasmid DNA, PCR product

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol

10×FlyCut® Buffer

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA


at -20℃ for two years

Kit Contents




FlyCut® BamHI

5,000 units

2×5,000 units

10×FlyCut® Buffer

2×1 ml

3×1 ml

10×DNA Loading Buffer

1 ml

1 ml

Literature Journal IF Author Date Link