Product Catalog

pEASY®-Blunt E2 Expression Kit

Catalog Number: CE211-01

Price:Please inquire first

Specification:
10 rxns

Product Details

pEASY®-Blunt E2 Expression Vector is constructed from pET vector. It utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.


•5 minutes fast ligation of Pfu-amplified PCR products.

•Ampicillin resistance.

•T7 promoter primer and T7 terminator primer for sequencing.

•Bacteriophage T7 lac promoter for high level expression.

•C-terminal 6xHis tag for easy purification.

•Trans 1-Tl Phage Resistant Chemically Competent Cell, high transformation efficiency (> 109 cfu/µg pUC 19 DNA) and fast growmg.

•E2 Expression Plasmid included as negative control.


Storage 

Trans 1-Tl Phage Resistant Chemically Competent Cell at -70°C for six months; others at -20°C for nine months.


Shipping

Dry ice (-70℃)

Product Composition

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References

Yang X , Du G , Yu Z , et al. A Novel NHERF1 Mutation in Human Breast Cancer and Effects on Malignant Progression[J]. Anticancer Research, 2017, 37(1):67.

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