The kit is designed to efficiently and quickly prepare DNA library from 1 ng to 1 μg double-stranded DNA for the Illumina high-throughput sequencing platform. The kit completes the DNA fragmentation, end repair and A-tailing in one step. The product can be directly used for adapter ligation without the purification steps. Due to the use of fragmentation enzymes to fragment the genome, libraries of inserts of different sizes can be obtained by adjusting the fragmentation time. And the kit is suitable for library construction of DNA from different sources and inputs.
Application
• Whole genome sequencing.
• Target gene sequencing.
• Exon sequencing / other targeted capture sequencing.
• Metagenomic sequencing.
Storage
at -20°C for one year
Shipping
Dry ice (-70℃)
| Component | KP231-01 (12 rxns) | KP231-02 (96 rxns) |
| Fragmentation Buffer (10×) | 60 μl | 480 μl |
| Fragmentation Enzyme Mix | 120 μl | 960 μl |
| Fragmentation Enhancer | 60 μl | 480 μl |
| TransNGS® Adapter for Illumina® (16 μM) | 60 μl | 480 μl |
| Adapter Dilution Buffer | 600 μl | 5 ml |
| Adapter-ligation Buffer for Illumina | 240 μl | 4×480 μl |
| Adapter-ligation Enzyme | 60 μl | 480 μl |
| TransNGS® Library Amplification SuperMix (2×) | 300 μl | 4×600 μl |
| TransNGS® Universal Primer Mix for Illumina* | 60 μl | 480 μl |
| Library Elution Buffer | 2 ml | 4×4 ml |
| Nuclease-free Water | 1 ml | 5 ml |
* The Adapter and Universal Primer in the kit are respectively suitable for short adapter and long adapter for library construction. Choose one according to requirments and do not use them at the same time.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
