Trans5α Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA).
Genotype
F-φ80d lacZΔM15 Δ(lacZYA-argF) U169 end A1 recA1 hsdR17 (rk-, mk+) supE44λ- thi-1 gyrA96relA1 phoA
Features
• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).
• Reduced recombination of cloned DNA.
• Blue/white selection.
Storage
Competent Cell at -70°C for six months; others at -20°C for one year
Shipping
Dry ice (-70℃)
Zhong S , Zhang S , Fan X , et al. A single-cell RNA-seq survey of the developmental landscape of the human prefrontal cortex[J]. Nature, 2018, 22, 555(7697):524-528.
Xu X , Xu J , Wu J , et al. Phosphorylation-Mediated IFN-γR2 Membrane Translocation Is Required to Activate Macrophage Innate Response[J]. Cell, 2018, 5, 175(5):1336-1351.
Han Y , Liu Q , Hou J , et al. Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression[J]. Cell, 2018, 19;173(3):634-648.
Chen K , Liu J , Liu S , et al. Methyltransferase SETD2-Mediated Methylation of STAT1 Is Critical for Interferon Antiviral Activity[J]. Cell, 2017, 170(3):492.
Liu S , Yu H , Liu Y , et al. Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis[J]. Molecular Cell, 2017, 7, 67(5):853-866.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
